Ciro Immobile, Felipe Caicedo and Luis Alberto lead the Lazio attack, as they must beat Stade Rennais and hope for a favour from Celtic.
It kicks off at Roazhon Park at 17.55 GMT.
Lazio still have a slim chance of reaching the Europa League Round of 32, but first need to beat Rennes and then hope Celtic also defeat Cluj in the other game.
This would put them on level points and with a superior head-to-head record against the Romanians.
Adam Marusic, Patric and Thomas Strakosha are out of action, while Jordan Lukaku and Andre Anderson are not on the Europa League squad list.
Silvio Proto therefore starts in goal, with Denis Vavro in defence, but otherwise it is a pretty strong line-up.
Above all, Luis Alberto is providing the support for Caicedo and Immobile upfront.
Rennes are already out of contention for qualification after one point from five games, having scored three goals and conceded eight.
Jakob Johansson is injured, while former Milan, Genoa and Torino striker Mbaye Niang is rested.
Rennes: Salin; Boey, Gnagnon, Nyamsi, Doumbia; Da Cunha, Grenier, Lea-Siliki, Tait; Gboho, Siebatcheu
Rennes bench: Mendy, Da Silva, Guitane, Niang, Morel, Camavinga, Del Castillo
Lazio: Proto; Bastos, Vavro, Acerbi; Lazzari, Parolo, Cataldi, Luis Alberto, Jony; Caicedo, Immobile
Lazio bench: Guerrieri, Luis Felipe, V Berisha, Correa, Lulic, Adekanye, Falbo
Ref: Jovanovic (SRB)
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