Atalanta need a victory to catch Roma in fourth place, so Duvan Zapata, Josip Ilicic and Papu Gomez face bottom of the table SPAL.
It kicks off at the Gewiss Stadium in Bergamo at 19.45 GMT. Click here for a match preview.
La Dea saw their excellent season so far suffer a blow last week with the Coppa Italia Round of 16 exit at the hands of Fiorentina.
Hans Hateboer is suspended and Timothy Castagne injured, so Remo Freuler moves over to keep Robin Gosens in his current role.
Atalanta need a victory to catch Roma in fourth place, so Duvan Zapata, Josip Ilicic and Papu Gomez face bottom of the table SPAL.
It kicks off at the Gewiss Stadium in Bergamo at 19.45 GMT. Click here for a match preview.
La Dea saw their excellent season so far suffer a blow last week with the Coppa Italia Round of 16 exit at the hands of Fiorentina.
Hans Hateboer is suspended and Timothy Castagne injured, so Remo Freuler moves over to keep Robin Gosens in his current role.
Mattia Caldara already made his ‘debut’ in the Coppa and is again used in the three-man defence after his return from 18 months at Milan.
Duvan Zapata is still trying to find full fitness after three months out with a serious thigh injury, supported by Ilicic and Gomez.
Marco Sportiello is surprisingly chosen in goal rather than Pierluigi Gollini.
SPAL are bottom of the Serie A table and the least prolific side in the division, with half their 12 goals contributed by ex-Atalanta centre-forward Andrea Petagna.
Since leaving Atalanta, Petagna has scored in each of his three Serie A games against the Bergamo boys, for a total four goals.
He leads the way with Federico Di Francesco, Gabriel Strefezza, new signing Bryan Dabo and another ex-Atalanta player, Arkadiusz Reca.
Coach Leonardo Semplici has to do without Dias Felipe, Marco D’Alessandro and Mohamed Fares.
Atalanta: Sportiello; Toloi, Caldara, Palomino; Freuler, De Roon, Pasalic, Gosens; Gomez; Duvan Zapata, Ilicic
SPAL: Berisha; Cionek, Vicari, Igor; Strefezza, Valoti, Missiroli, Dabo, Reca; Di Francesco, Petagna
Ref: La Penna