Nicolò Zaniolo, Henrikh Mkhitaryan and Edin Dzeko lead the Roma attack, with Bryan Cristante in midfield, while Sevilla choose Lucas Ocampos, Suso and Youssef En-Nesyri.
The Europa League Round of 16 is a one-off knockout round for a place in the quarter-finals.
It kicks off on neutral turf in Duisburg at 17.55 UK time (16.55 GMT).
The winners will be decided after 90 minutes, extra time or even penalties and go on to face Wolves or Olympiakos in the quarter-finals.
Zaniolo has only started one match since suffering a torn ACL in January, last week’s 3-1 Serie A victory over Juventus, but did seem to be in sparkling form with a strong run from midfield to set up Diego Perotti’s goal.
Coach Paulo Fonseca has revitalised Roma since moving to a 3-4-2-1 formation, so Zaniolo partners Mkhitaryan in support of Dzeko.
Lorenzo Pellegrini fractured his nose last week and is available to play with a protective mask, but is only on the bench to begin with, as Cristante steps in for suspended Jordan Veretout.
Chris Smalling and Davide Zappacosta have seen their loan spells terminated, so Aleksandar Kolarov and Roger Ibanez complete the three-man defence.
Javier Pastore, Mert Cetin and Juan Jesus were left off the UEFA squad list.
Sevilla are the Europa League specialists, winning this trophy three years in a row from 2014-16, and ended the Liga season on strong form too.
Nemanja Gudelj is out of action after testing positive for COVID-19 on July 26, but it has not spread to the rest of his squad.
Tomas Vaclik was expected to return from injury in goal, but it’s Yassine Bounou who gets the gloves, with Fernando coming into midfield for Gudelj.
Ocampos has been in great shape lately and he is chosen with ex-Milan winger Suso to complete the trident attack.
Surprisingly, it’s not with Luuk de Jong or Munir El Haddadi, but rather Youssef En-Nesyri.
Ocampos is their top scorer this season with 16 goals in 40 competitive games, while Munir has 10 in 28 matches, De Jong following on seven.
En-Nesyri has found the net five times in 22 appearances for Sevilla this season, joining from Leganes in January for €20m.
Roma would have reason for being distracted, as this morning the club announced a successful takeover bid from The Friedkin Group.
Sevilla: Bounou; Jesus Navas, Koundé, Diego Carlos, Reguilòn; Banega, Fernando, Jordan; Ocampos, En-Nesyri, Suso
Sevilla bench: Vaclik, Sanchez, Sergi Gomez, Munir, Escudero, L de Jong, Oliver Torres, Vazquez, Mena, Jose Alonso, Genaro, Perez Rico
Roma: Pau Lopez; Mancini, Ibanez, Kolarov; Bruno Peres, Cristante, Diawara, Spinazzola; Zaniolo, Mkhitaryan; Dzeko
Roma bench: Mirante, Cardinali, L Pellegrini, Perotti, Villar, Under, Santon, Kalinic, Fazio, C Perez, Calafiori, Kluivert
Ref: Kuipers (NED)