Ciro Immobile leads Italy out against the Netherlands with a revamped defence, while Memphis Depay is joined by Luuk de Jong.
It kicks off at the Gewiss Stadium in Bergamo at 19.45 UK time (18.45 GMT).
There are several changes from the side that drew 0-0 in Poland on Sunday evening, starting with the centre-forward.
Andrea Belotti is suspended, but was always going to step aside for Lazio hitman Immobile.
Federico Chiesa and Lorenzo Pellegrini keep their spots in the trident attack, though, while the midfield is also unchanged.
Giorgio Chiellini returns in defence, with Danilo D’Ambrosio and Leonardo Spinazzola taking the full-back roles.
Stephan El Shaarawy has been given the all-clear for a spot on the bench after it was ascertained he had posted a ‘false positive’ COVID-19 test.
Holland are fresh from their own 0-0 draw with Bosnia-Herzegovina and have failed to score in their last three matches between Nations League and friendly with Mexico.
Inter defender Stefan de Vrij and Atalanta wing-back Hans Hateboer are among the familiar faces, although Marten de Roon is suspended following his yellow card with Bosnia.
Luuk de Jong is the centre-forward with Memphis Depay and Georginio Wijnaldum, bringing Danny Blind into midfield.
The system isn’t very clear, but could be a 5-3-2 just as much as a 3-5-2, which either way is revolutionary for the Dutch and Frank de Boer.
There is no VAR in the Nations League group phase, but five substitutions are allowed.
Italy: G Donnarumma; D'Ambrosio, Bonucci, Chiellini, Spinazzola; Barella, Jorginho, Verratti; Chiesa, Immobile, Pellegrini
Italy bench: Sirigu, Cragno, Mancini, Caputo, Berardi, Kean, Sensi, Biraghi, Acerbi, Florenzi, Locatelli, El Shaarawy
Netherlands: Cillessen; Hateboer, de Vrij, Van Dijk, Ake, Blind; Van de Beek, F de Jong, Wijnaldum; L de Jong, Depay
Netherlands bench: Krul, Bizot, Veltman, Berghuis, Babel, Promes, Wijndal, Koopmeiners, Malen, Strootman, Stengs, Dumfries
Ref: Taylor (ENG)
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