Napoli could replace Lorenzo Insigne with Eljif Elmas and have Dries Mertens supporting Andrea Petagna in attack against HNK Rijeka.
The match starts at 17.55 UK time.
The Partenopei started the Europa League with a poor performance and a 1-0 defeat to AZ Alkmaar in the first round but bounced back with a 1-0 win against Real Sociedad last time.
Gattuso’s men arrive from another setback in Serie A against Sassuolo at the weekend and travel to Croatia looking to get back on track.
The Azzurri are expected to continue with a 4-2-3-1 formation, with Mertens right behind Petagna in attack.
Elmas could start for the bruised Insigne and Nikola Maksimovic is expected to start over Kostas Manolas at the back.
Diego Demme and Stanislav Lobotka will get another chance in place of Tiemoue Bakayoko and Fabian Ruiz.
The home side have lost both games and only scored once in their first two matches, coming from a heavy 4-1 defeat to AZ in Holland last time.
Rijeka continued with a 2-0 defeat to Sibenik in their domestic League too and are desperate for a good experience against the Partenopei at home.
Rijeka (probable): Nevistic; Tomecak, Galovic, Velkovski, Escoval, Stefulj; Menalo, Pavicic, Andrijasevic, Loncar; Kulenovic
Napoli (probable): Meret; Di Lorenzo, Maksimovic, Koulibaly, Mario Rui; Demme, Lobotka; Politano, Mertens, Elmas; Petagna
Referee: Gestranius (FIN)