Federico Fazio, Lorenzo Pellegrini and Davide Santon have tested positive for Covid-19, according to reports in Italy.
The former Tottenham star was on the bench in Roma's 3-1 away win over Genoa on Sunday while Pellegrini started the game and Santon was injured.
Pellegrini revealed he is positive for Coronavirus with an Instagram posts where he says he has mild symptoms.
Roma's captain Edin Dzeko had tested positive for Covid last week alongside Primavera goalkeeper Pietro Boer.
Amadou Diawara and Riccardo Calafiori are still positive but have had successful medical tests and have begun to train individually.