Sergej Milinkovic-Savic returns to the starting XI with Ciro Immobile and Pepe Reina, but Borussia Dortmund are without Erling Haaland and drop Jadon Sancho against Lazio.
It kicks off at the Signal Iduna Park at 20.00 GMT, you can follow all the build-up and action as it happens from this game and Juventus-Dynamo Kiev on the LIVEBLOG.
The Biancocelesti are unbeaten in Europe this season, despite an injury and COVID crisis, and sit just one point behind group leaders Borussia Dortmund.
A victory would not only secure qualification to the final 16 for the first time in 20 years, but also send them into first place.
Milinkovic-Savic has now fully recovered from COVID-19 and is in the starting XI with Luis Alberto, Joaquin Correa and ex-Dortmund striker Immobile.
Luiz Felipe is still only fit for the bench, so Patric starts with Wesley Hoedt and Francesco Acerbi, while Reina gets the nod in goal rather than Thomas Strakosha.
Vedat Muriqi is still on the treatment table.
Borussia Dortmund are without Dan Axel Zagadou, Reinier and long-term absentee Marcel Schmelzer, but the big news is that hitman Haaland is not even on the bench after a hamstring injury in training yesterday.
Haaland is only 20 years old, but already a European superstar with 17 goals in 14 competitive games this season.
That includes six in just four Champions League outings, a goal every 58 minutes in this competition.
Sky Sport Italia report he could be out for four to six weeks.
Jadon Sancho and Axel Witsel are also dropped, as Borussia Dortmund transform with Marco Reus as a False 9.
Lazio beat Borussia Dortmund 3-1 at the Stadio Olimpico in the opening fixture and would relish a repeat, especially just days after their surprise 3-1 Serie A home loss to Udinese.
Borussia Dortmund: Burki; Piszczek, Akanji, Hummels; Morey, Bellingham, Delaney, Guerreiro; Reyna, Reus, T Hazard
Borussia Dortmund bench: Unbehaun, Hitz, Zagadou, Sancho, Dahoud, Schulz, Moukoko, Brandt, Witsel, Passlack
Lazio: Reina; Patric, Hoedt, Acerbi; Marusic, Milinkovic-Savic, Leiva, Luis Alberto, Fares; Correa, Immobile
Lazio bench: Strakosha, Alia, Luiz Felipe, Pereira, Anderson, Parolo, Escalante, Caicedo, Radu, Lazzari, Cataldi, Akpa Akpro
Ref: Lahoz (ESP)