Atalanta look to Luis Muriel, Duvan Zapata and Matteo Pessina in the battle for a top four spot with a Napoli side still decimated by injuries.
It kicks off in Bergamo’s Gewiss Stadium at 17.00 GMT.
These sides are locked together on 40 points going into the weekend, although Napoli have a game in hand to be played against Juventus.
They managed an extraordinary 1-0 win over the Bianconeri last week, although it was a rare gem in a 2021 dominated by misfortune.
Gennaro Gattuso made the Europa League trip to Granada on Thursday night with only 12 senior outfield players available and that number has increased to 14, because Kalidou Koulibaly and Faouzi Ghoulam recovered from COVID-19, but aren’t fit to start.
Still out of action are Hirving Lozano, Dries Mertens, Andrea Petagna, Kostas Manolas, Diego Demme, Elseid Hysaj and David Ospina.
Squad rotation options are practically non-existent, so exhaustion could be a factor, considering Napoli only played in Spain on Thursday.
Atalanta, on the other hand, had a full week to prepare for this game and their upcoming Champions League Round of 16 clash with Real Madrid.
Hans Hateboer is the only absentee, although Joakim Maehle is carrying a knock.
Duvan Zapata is supported by Luis Muriel and Matteo Pessina, where La Dea hope to replicate their 3-1 Coppa Italia semi-final victory over Napoli here just a couple of weeks ago.
Napoli have won just one of their last six competitive meetings with Atalanta, drawing two and losing three.
Atalanta: Gollini; Toloi, Romero, Djimsiti; Maehle, De Roon, Freuler, Gosens; Pessina; Muriel, Duvan Zapata
Napoli: Meret; Di Lorenzo, Maksimovic, Rrahmani, Mario Rui; Fabian Ruiz, Bakayoko, Zielinski; Elmas, Osimhen, Politano
Ref: Di Bello