Roma rely on Edin Dzeko, Lorenzo Pellegrini and Jordan Veretout for their Europa League quarter-final, while Ajax look to Dusan Tadic and Ryan Gravenberch.
The first leg kicks off in Amsterdam at 20.00 UK time (19.00 GMT).
Both teams have numerous injury problems going into the quarter-finals.
The Giallorossi leave behind Chris Smalling, Henrikh Mkhitaryan, Stephan El Shaarawy, Marash Kumbulla and suspended Rick Karsdorp.
The good news is the return of Veretout from the start after over a month out, while Pedro partners Lorenzo Pellegrini in support of Dzeko.
Borja Mayoral drops to the bench with Carles Perez, while Amadou Diawara gets the midfield slot rather than Gonzalo Villar.
Ajax are forced to use third choice goalkeeper Kjell Scherpen, as Andre Onana is suspended for doping violations and former Roma shot-stopper Maarten Stekelenburg picked up a knee injury at the weekend.
Daley Blind is a big loss after spraining his ankle on international duty, while January signing Sebastien Haller was accidentally left off the UEFA squad and is therefore ineligible.
That means Dusan Tadic leads the way with support from Antony and David Neres, while Gravenberch is one to watch, considered an heir to Paul Pogba.
Ajax: Scherpen; Rensch, J Timber, Martinez, Tagliafico; Klaassen, Alvarez, Gravenberch; Antony, Tadic, Neres
Ajax bench: Kotarski, Reiziger, Klaiber, Idrissi, Ekkelenkamp, Kudus, Traore, Taylor, Brobbey, Kasanwirjo, Q Timber
Roma: Pau Lopez; Mancini, Cristante, Ibanez; Bruno Peres, Diawara, Veretout, Spinazzola; Pellegrini, Pedro; Dzeko
Roma bench: Mirante, Fuzato, Villar, Santon, Mayoral, Perez, Ciervo, Calafiori, Milanese
Ref: Karasev (RUS)