Atalanta need a victory to retain fourth place and go all-out attack against Fiorentina, who start Christian Kouame with Dusan Vlahovic.
It kicks off at the Stadio Franchi at 19.45 UK time (18.45 GMT).
This is an historic occasion, as it will be the first ever Serie A game to be shown live on one of the main U.S. broadcast networks, showing on ABC tonight.
Atalanta need a win to hold onto fourth place, as Juventus and Napoli both won today, as did Milan yesterday, keeping the race for a Champions League spot wide open.
Matteo Pessina was one of the many Italy internationals who caught COVID-19 last week, so he is ruled out along with injured Hans Hateboer and Bosko Sutalo.
Gian Piero Gasperini goes all-out attack with Ruslan Malinovskyi, Mario Pasalic, Luis Muriel and Duvan Zapata together.
That is possible because Gasp is again experimenting with a four-man defence.
Fiorentina aren’t entirely safe from relegation yet, despite improving form recently, but they will want to step it up with President Rocco Commisso in the stands.
Franck Ribery and Erick Pulgar are suspended, with Kevin Malcuit, Igor and Aleksandr Kokorin injured.
Christian Kouame is chosen to partner Vlahovic in a more attack-minded approach than other alternative Valentin Eysseric.
There’s room for veteran Giacomo Bonaventura and temperamental Italy international Gaetano Castrovilli in midfield.
Fiorentina: Dragowski; Milenkovic, Pezzella, Martinez Quarta; Caceres, Bonaventura, Amrabat, Castrovilli, Biraghi; Kouame, Vlahovic
Fiorentina bench: Terracciano, Rosati, Borja Valero, Maxi Olivera, Venuti, Malcuit, Barreca, Montiel, Ponsi, Munteanu, Callejon, Eysseric
Atalanta: Gollini; Toloi, Romero, Palomino, Gosens; De Roon, Freuler; Malinovskyi, Pasalic, Muriel; Duvan Zapata
Atalanta bench: Rossi, Sportiello, Maehle, Sutalo, Lammers, Caldara, Djimsiti, Kovalenko, Ruggeri, Miranchuk, Ilicic