Juventus launch Carlos Tevez, Fernando Llorente and Paul Pogba against Fiorentina’s Mario Gomez, Juan Cuadrado and Alberto Aquilani.
The bitter Serie A grudge match kicks off at 11.30 GMT, click here for a match preview.
These clubs have never seen eye to eye and the rivalry is only intensifying this season, as the Viola fought back from 2-0 down for a 4-2 victory, becoming the only team to beat Juve in the current campaign.
They will face off again on March 13 and 20 in the Europa League Round of 16, so this is also a taster of what is to come in that competition.
Juventus soared 11 points clear at the top of the Serie A table, albeit with Roma having a game in hand, while Fiorentina are in fourth and fighting for a Champions League spot.
Andrea Pirlo is suspended with Mirko Vucinic and Simone Pepe injured, but Giorgio Chiellini returns to the starting XI and Leonardo Bonucci is rested.
Claudio Marchisio takes over the deep-lying playmaker role and Arturo Vidal is back from suspension with Pogba.
Borja Valero’s four-match ban was cut to three on appeal, though that still rules him out of this clash along with Nenad Tomovic.
Giuseppe Rossi and Ante Rebic are on the injury list, but Gomez is getting back to full fitness after five months out with a knee injury.
He is flanked by Juan Guillermo Cuadrado and Juan Manuel Vargas, while former Manchester United midfielder Anderson starts with David Pizarro and ex-Juve man Aquilani.
Fiorentina have a poor record in Turin, winning only one of their last 21 visits, a 3-2 result in March 2008.
The Tuscans haven’t kept a clean sheet here since November 1981, conceding in the next 27 games.
Juventus: Buffon; Barzagli, Ogbonna, Chiellini; Lichtsteiner, Vidal, Marchisio, Pogba, Asamoah; Tevez, Llorente
Juventus bench: Storari, Rubinho, Caceres, Bonucci, Peluso, Isla, Padoin, Giovinco, Quagliarella
Fiorentina: Neto; Diakitè, Gonzalo Rodriguez, Savic, Pasqual; Anderson, Pizarro, Aquilani; Cuadrado, Gomez, Vargas
Fiorentina bench: Rosati, Lupatelli, Roncaglia, Compper, Bakic, Mati Fernandez, Ambrosini, Wolski, Joaquin, Matos, Matri, Ilicic
See the latest Serie A predictions and betting tips with Eurotips.co.uk