Cesena have the upper hand in the promotion play-off after beating Latina 2-1 in the first leg.
Palermo and Empoli have already secured their places in Serie A for next season and will be joined by one of these two clubs.
Cesena played the first leg on home turf and went 2-0 up through Volta and Marilungo.
However, Latina fought hard in the final 30 minutes and got one back right at the death thanks to Cisotti.
Away goals do not count in the play-offs, so Latina will need to win by two clear goals on Wednesday.
Cesena 2-1 Latina
Scorers: Volta 45 (C), Marilungo 52 (C), Cisotti 90 (L)
See the latest Serie A predictions and betting tips with Eurotips.co.uk