Zenit, Maribor, BATE Borisov, APOEL and Porto are through to the Champions League, which is bad news for Roma.
The second legs of some play-offs were played this evening with some truly surprising results.
Celtic, who were given another chance in the tournament by Legia Warsaw fielding an inelgible player in the last round, suffered a shock 1-0 home defeat to Slovenians Maribor.
The Scottish champions crashed out 2-1 on aggregate.
Zenit crushed Standard Liege 3-0 despite going down to 10 men and that 4-0 aggregate victory is bad news for Roma.
As Zenit go into the draw for the group phase on Friday, Roma slip down to fourth seeds.
BATE Borisov made short work of Slovan Bratislava, winning 3-0 on the night, 4-1 on aggregate.
Cypriots APOEL demolished AaB 4-0 after a 1-1 first leg draw.
Jackson Martinez was on target as Porto comfortably beat Lille 2-0, following on from their 1-0 victory in France.
See the latest Serie A predictions and betting tips with Eurotips.co.uk