Andre Dias has failed a fitness test and is not in Lazio's squad for the Derby della Capitale.
Coach Edy Reja had hinted he would be ready for Sunday afternoon's game, but the Brazilian defender did not reassure the medical staff.
Also out of the clash with Roma are Tommaso Rocchi and Mobido Diakitè.
However, Giuseppe Sculli is included after he was forced into a substitution against Palermo last week due to an allergic reaction.
Lazio squad: Muslera, Berni; Biava, Lichtsteiner, Radu, Scaloni, Stendardo, Garrido; Bresciano, Brocchi, Gonzalez, Hernanes, Ledesma, Matuzalem, Mauri; Floccari, Kozak, Sculli, Zarate
See the latest Serie A predictions and betting tips with Eurotips.co.uk