Milan start with Robinho, Stephan El Shaarawy and Kevin-Prince Boateng against a reshuffled Sampdoria.
The Rossoneri begin their Serie A campaign at San Siro against the newly-promoted Blucerchiati at 17.00 UK time.
Alexandre Pato is once again on the injury list with a thigh problem, joined by Ignazio Abate, Massimo Ambrosini, Sulley Muntari, Rodney Strasser and Didac Vila.
New signing Giampaolo Pazzini is in the squad just days after exchanging places with Antonio Cassano at Inter, but he is on the bench to begin with.
Sampdoria make their return to Serie A after a year in the wilderness and have ex-Italy Under-21 Coach Ciro Ferrara in charge.
He has to do without the suspended Nicola Pozzi and Da Costa for this opening game, plus injured Juan Antonio.
Maxi Lopez is back at San Siro to face his old club, but is suffering from back pain, so only has a spot on the new elongated bench.
As of this season, teams can have up to 12 players available for substitutions, though only three can actually come on to the field.
The setbacks mean Samp start with Eder, Nenad Krsticic and former Juventus winger Marcelo Estigarribia in a 4-3-3 system.
Milan: Abbiati; De Sciglio, Bonera, Yepes, Antonini; Flamini, Montolivo, Nocerino; Boateng, El Shaarawy; Robinho
Sampdoria: Romero; Berardi, Gastaldello, Rossini, Costa; Obiang, Tissone, Poli; Estigarribia, Eder, Krsticic