The Tuscan Derby is set to be a fiery affair between a Siena side fighting relegation and Fiorentina chasing the Champions League.
It all kicks off at the Stadio Artemio Franchi Montepaschi di Siena at 19.45 UK time (18.45 GMT). Click here for a match preview.
Adem Ljajic begins a two-match ban for insulting the referee after the Viola’s home defeat to Roma, joining Nenad Tomovic on the naughty step.
Alberto Aquilani, Momo Sissoko and Michele Camporese are sidelined, but Giuseppe Rossi is on the bench for the first time since his devastating knee injury for Villarreal in October 2011.
David Pizarro is surprisingly rested, so two of the three usual midfielders are absent, while Marcelo Larrondo replaces Ljajic.
Siena are coming off three consecutive defeats without scoring, a run that has plunged them into the bottom two, five points adrift of safety.
Dias Felipe sits out a ban, so Claudio Terzi comes into defence.
Siena: Pegolo; Teixeira, Terlizzi, Terzi; Angelo, Calello, Vergassola, Bolzoni, Rubin; Reginaldo, Emeghara
Siena bench: Farelli, Uvini, Valiani, Paolucci, Verre, Agra, Paci, Rosina, Mannini, Sestu, Bogdani, Grillo
Fiorentina: Viviano; Savic, Rodriguez, Compper; Savic, Migliaccio, Fernandez, Borja Valero, Pasqual; Cuadrado, Jovetic, Larrondo
Fiorentina bench: Neto, Hegazi, Roncaglia El Hamdaoui, Pizarro, Lupatelli, Llama, Capezzi , Wolski, Toni, Rossi, Romulo
Think you know your Italian football? Share your knowledge, tips and comments to win cash prizes in OLBG's tipster competition - £5,000 monthly.