Fiorentina aim to continue their remarkable form with Giuseppe Rossi against a Sampdoria side in crisis.
It kicks off at the Stadio Franchi at 19.45 GMT, click here for a match preview.
The Viola are in sparkling form despite Mario Gomez’s long-term absence due to a knee injury.
In the last eight days they won 2-0 away to Milan and maintained their 100 per cent Europa League record.
Massimo Ambrosini is still out of action, while David Pizarro is only fit for the bench.
Delio Rossi returns to face his former club, having famously been fired after punching Adem Ljajic in the dugout.
Daniele Gastaldello and Andrea Costa are suspended for this fixture, as Rossi is hanging by a thread following the shock 4-3 home defeat to Sassuolo.
Fiorentina: Neto; Pasqual, Savic, Rodriguez, Tomovic; Matias Fernandez, Borja Valero, Aquilani; Cuadrado, Rossi, Joaquin
Fiorentina bench: Munua, Ronacaglia, Compper, Alonso, Vecino, Vargas, Pizarro, Wolski, Olivera, Ilicic, Rebic, Matos
Sampdoria: Da Costa; Mustafi, Palombo, Regini, De Silvestri; Obiang, Kristicic, Gentsoglou; Berardi, Gabbiadini, Eder
Sampdoria bench: Fiorillo, Tozzo, Gavazzi, Fornasier, Poulsen, Renan, Eramo, Soriano, Bjarnason, Pozzi, Wszolek, Petagna