Juan Cuadrado’s Colombia take on Edinson Cavani’s Uruguay in a World Cup match with a lot of Serie A representation.
It kicks off in Rio de Janeiro at 21.00 UK time (20.00 GMT) with only one able to continue this campaign. The winners will go on to face Brazil in the quarter-final after the hosts beat Chile on penalties.
This is only the second time these nations have faced off in a World Cup, the other being in 1962 when Uruguay fought back from a goal down to win 2-1. However, that was a group phase game and neither of them progressed to the knockouts.
Los Cafeteros went through their group with a 100 per cent record and rested the likes of Atalanta defender Mario Yepes, Cristian Zapata of Milan, Teofilo Gutierrez and Carlos Sanchez for the 4-1 win over Japan.
James Rodriguez has emerged as the breakout star along with Fiorentina wide man Cuadrado.
Pablo Armero and Juan Camilo Zuniga of Napoli are also in the squad with Jackson Martinez, leaving pacey Cagliari striker Victor Ibarbo on the bench.
Uruguay must do without their top scorer Luis Suarez, who received a four-month ban for biting Italy defender Giorgio Chiellini.
Diego Forlan therefore returns to the starting XI alongside ex-Napoli forward Cavani, while Maxi Pereira is available after his suspension.
La Celeste also have numerous Serie A representatives in their team, including Juventus defender Martin Caceres, Alvaro Gonzalez of Lazio and Palermo midfielder Arevalo Rios.
Colombia: Ospina; C Zuniga, C Zapata, Yepes, Armero; Aguilar, C Sanchez; Cuadrado, James Rodriguez, Jackson Martinez; Gutierrez
Uruguay: Muslera; Caceres, Godin, Gimenez, A Pereira; Gonzalez, Arevalo Rios, Rodriguez, Maxi Pereira; Cavani, Forlan
Betting on Serie A? Bet365 are offering a 100% deposit bonus, up to £200 - find out more