Ascoli have been docked another point for financial irregularities, meaning they are now at -4.
The club had already been ‘fined' three points after failing to provide the right paperwork when registering for the new season.
However, the FIGC won its appeal to the Court of Federal Justice to have another point removed from their tally.
Ascoli are now in penultimate place in the Serie B table with 15 points.
There was a similar incident in Serie A today, when Bologna were docked a point for failing to cover unpaid wages in the summer.