David Pizarro has failed a fitness test, while Marco Borriello starts in Roma's early kick-off against Udinese.
Serie A Week 13 begins at 17.00 UK time at the Stadio Olimpico and both sides have lost players due to international duty.
Daniele De Rossi limped off with a groin strain during Italy's 1-1 draw with Romania on Wednesday and is out of action along with John Arne Riise, Rodrigo Taddei, Simone Perrotta and Pizarro.
The Chilean was expected to make his comeback, but failed a last-minute fitness test so will only be on the bench.
In attack Borriello partners Francesco Totti and Jeremy Menez, leaving Mirko Vucinic out, but Julio Sergio has recovered from a muscular problem to play in goal.
Udinese also had problems on international duty, as Chilean Alexis Sanchez is not fit enough to feature, having only returned from South America on Friday afternoon.
This means they switch to a 3-5-2 system with Antonio Di Natale and Antonio Floro Flores leading the way.
Giampiero Pinzi and Giovanni Pasquale return from suspension for the Friulani.
Roma: Julio Sergio; Cassetti, Juan, N Burdisso, Castellini; Brighi, Simplicio, Greco; Menez; Totti, Borriello
Roma bench: Lobont, Cicinho, Mexes, Pizarro, Baptista, Adriano, Vucinic
Udinese: Handanovic; Benatia, Coda, Domizzi; Isla, Pinzi, Inler, Asamoah, Armero; Di Natale, Floro Flores
Udinese bench: Belardi, Sanchez, Corradi, Denis, Badu, Pasquale, Angella
Tickets for the Champions League final between Juventus and Real Madrid are still on sale with reseller Viagogo - Click to find out more