Juventus field a whole new 3-4-2-1 system against Barcelona following Giorgio Chiellini’s injury, but Leo Messi is surprisingly benched.
It kicks off in Turin at 19.45 GMT, follow the build-up and action as it happens from this game and Atletico Madrid-Roma on the LIVEBLOG.
The Bianconeri would go through with a victory this evening, although a draw would be enough if Sporting fail to beat Olympiacos.
Giorgio Chiellini, Federico Bernardeschi and Marko Pjaca are the injury absentees for Max Allegri, while Stephan Lichtsteiner is cup-tied, but Gigi Buffon and Andrea Barzagli return after they were rested for Sunday’s 3-2 defeat to Sampdoria.
They were still smarting from Italy’s World Cup play-off loss with Sweden, a game that effectively ended their international careers.
Chiellini was a late addition to the list after pulling up with a thigh problem in training, so Allegri has decided to transform the side in a new 3-4-2-1 formation.
Douglas Costa and Paulo Dybala support centre-forward Gonzalo Higuain, with Juan Cuadrado and Alex Sandro as wing-backs.
All eyes will be on Dybala, who hasn’t scored in the Champions League since the 3-0 quarter-final victory here over Barcelona in April, a dry run of eight games.
Barcelona would be content with a point in Turin and the memories of last season’s 3-0 quarter-final defeat here still burns.
Javier Mascherano, Arda Turan, Rafinha, Sergi Roberto, Andre Gomes and Dembele are all out of action.
The real shock is that Lionel Messi is left on the bench, so former Milan man Gerard Deulofeu is the partner to Luis Suarez with Paulinho.
Juventus: Buffon; Rugani, Barzagli, Benatia; Cuadrado, Pjanic, Khedira, Alex Sandro; Dybala, Douglas Costa; Higuain
Juventus bench: Szczesny, De Sciglio, Marchisio, Matuidi, Asamoah, Bentancur, Mandzukic
Barcelona: Ter Stegen; Semedo, Pique, Umtiti, Digne; Sergio Busquets, Rakitic, Iniesta; Deulofeu, L Suarez, Paulinho
Barcelona bench: Cillessen, D Suarez, Messi, Alcacer, Alba, Aleix Vidal, Vermaelen
Ref: Mazic (SER)
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