Roma are expected to be without Javier Pastore, Daniele De Rossi and Diego Perotti for the Champions League game with Viktoria Plzen.
The Giallorossi notched up a second consecutive Serie A victory yesterday, beating Frosinone and then Lazio 3-1.
Pastore limped off during the first half with a calf injury and is not expected to be fit for at least the next two matches against Viktoria Plzen and Empoli.
More tests are needed to ascertain the extent of the damage.
De Rossi also asked to be substituted, as he was suffering from the inflammation of his knee.
“I’m trying to control the situation, but I do need some rest,” confirmed the captain to Il Tempo newspaper.
Aleksandar Kolarov should still play in the Champions League on Tuesday despite a surprise setback.
It was revealed after the Rome Derby that Kolarov had fractured the little toe on his left foot, but played on through the game anyway and even scored a goal.
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