Fiorentina focus on Federico Chiesa and Luis Muriel, while Napoli go with Dries Mertens and Lorenzo Insigne.
It kicks off at the Stadio Franchi at 17.00 GMT, you can follow the build-up and action from this game and Parma-Inter on the LIVEBLOG.
The Partenopei begin life after Marek Hamsik, as their captain is not even in the squad ahead of his €20m move to Dalian Yifang.
Defender Raul Albiol is out with a knee tendon issue and in doubt for the Europa League clash with FC Zurich on Thursday evening too.
Arkadiusz Milik is not 100 per cent fit either, so Dries Mertens and Lorenzo Insigne lead the way upfront, with Fabian Ruiz replacing Hamsik in midfield.
Insigne also takes over the captain’s armband, becoming the first Neapolitan to lead out his hometown club since Paolo Cannavaro.
Nikola Maksimovic steps in at centre-back, giving the full-back roles to Elseid Hysaj and Mario Rui.
Fiorentina have issues of their own to deal with, as Marco Benassi and Nikola Milenkovic are suspended, with Vincent Laurini injured.
Vitor Hugo is in the squad, but not fully fit and only has a place on the bench.
That leaves few alternatives in defence, so David Hancko and Federico Ceccherini get rare starts.
Muriel has been on fire since he arrived from Sevilla and leads the attack with support from Federico Chiesa and Gerson, leaving both Giovanni Simeone and Kevin Mirallas as options to come off the bench.
Fiorentina: Lafont; Ceccherini, Pezzella, Hancko, Biraghi; Dabo, Edimilson Fernandes, Veretout; Chiesa, Muriel, Gerson
Fiorentina bench: Terracciano, Brancolini, Norgaard, Simeone, Pjaca, Mirallas, Graiciar, Antzoulas, Vitor Hugo
Napoli: Meret; Hysaj, Maksimovic, Koulibaly, Mario Rui; Callejon, Allan, Fabian Ruiz, Zielinski; Insigne, Mertens
Napoli bench: Ospina, Karnezis, Chiriches, Malcuit, Ghoulam, Luperto, Diawara, Ounas, Verdi, Milik