Fiorentina look to Kevin-Prince Boateng and Federico Chiesa, as Inter must win to maintain their status as Serie A leaders.
It kicks off at the Stadio Artemio Franchi at 19.45 GMT.
Juventus beat Udinese 3-1 this afternoon and temporarily recaptured top spot, while Inter need to shake off the disappointment of their midweek Champions League exit.
It remains an emergency situation for Antonio Conte, who is still without Antonio Candreva, Stefano Sensi, Nicolò Barella, Roberto Gagliardini, Kwadwo Asamoah and Alexis Sanchez.
Diego Godin is rested in favour of Alessandro Bastoni, but ex-Fiorentina men Matias Vecino and Borja Valero start behind Romelu Lukaku and Lautaro Martinez.
Fiorentina have lost four Serie A matches in a row and there are reports Vincenzo Montella could be sacked if he capitulates again this evening.
Franck Ribery has been ruled out for at least 10 weeks following ankle surgery, so Kevin-Prince Boateng is chosen rather than Dusan Vlahovic to partner Chiesa upfront.
Henrique Dalbert will be eager to make his mark, as he is on loan from Inter, while German Pezzella returns with the aid of a protective mask after fracturing his cheekbone.
Fiorentina: Dragowski; Milenkovic, Pezzella, Caceres; Lirola, Pulgar, Badelj, Castrovilli, Dalbert; Chiesa, Boateng
Inter: Handanovic; Skriniar, De Vrij, Bastoni; D'Ambrosio, Vecino, Brozovic, Borja Valero, Biraghi; R Lukaku, Lautaro Martinez
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