Davide Massa will officiate the Coppa Italia quarter-final between Napoli and Lazio, while Gianluca Rocchi takes Juventus-Roma.
The quarter-finals of the tournament begin this week, with Torino-Milan on January 28 and Inter-Fiorentina on January 29.
Massa has been assigned to the Stadio San Paolo, where Napoli host trophy holders Lazio on Tuesday at 19.45 GMT.
He will be aided by Luigi Nasca in the VAR booth.
Juventus-Roma kicks off on Wednesday at 19.45 GMT, with Rocchi holding the whistle in Turin.
His Video Assistant Referee will be Luca Banti.
The winners of the Napoli-Lazio game go on to face Fiorentina or Inter in the two-legged semi-final.
Juventus or Roma will then take on Milan or Torino in the other showdown.
The Coppa Italia Final is set for May 13 at the Stadio Olimpico in Rome.
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