Inter start Alexis Sanchez, Romelu Lukaku and Lautaro Martinez together with Ashley Young, with Christian Eriksen on the bench, as Fiorentina rely on Dusan Vlahovic and Federico Chiesa.
The Coppa Italia quarter-final kicks off at San Siro at 19.45 GMT.
A winner will be decided after 90 minutes, extra time or penalties, with the victor going on to face Napoli in the two-legged semi-final.
The pressure is growing on Inter after three consecutive Serie A stalemates, although they are just three points off leaders Juventus.
Inter start Alexis Sanchez, Romelu Lukaku and Lautaro Martinez together with Ashley Young, with Christian Eriksen on the bench, as Fiorentina rely on Dusan Vlahovic and Federico Chiesa.
The Coppa Italia quarter-final kicks off at San Siro at 19.45 GMT.
A winner will be decided after 90 minutes, extra time or penalties, with the victor going on to face Napoli in the two-legged semi-final.
The pressure is growing on Inter after three consecutive Serie A stalemates, although they are just three points off leaders Juventus.
They have gone out of the Coppa Italia in the quarter-final stage for the last three seasons running.
Kwadwo Asamoah, Marcelo Brozovic and Roberto Gagliardini are injured, but there is no rest for Romelu Lukaku, who starts in a new 3-4-1-2 formation with Lautaro Martinez, moving Alexis Sanchez apparently to a trequartista role.
Lautaro has picked up a two-match Serie A ban for his furious reaction to the referee during a 1-1 draw with Cagliari, so has no need for rest.
Ashley Young provided an assist for Lautaro’s goal on his Serie A debut and plays again, albeit this time on the left, as Antonio Candreva is available again after suspension.
Borja Valero takes the midfield reins against his former club and even Matias Vecino returns, after Everton failed to meet the €20m asking price.
Fiorentina are unbeaten since Beppe Iachini took over from Vincenzo Montella during the Christmas break.
However, the squad is short for this trip, as Gaetano Castrovilli clearly isn’t available after he was kept in hospital for two nights following a dizzy spell and knock to the head on Saturday against Genoa.
Kevin-Prince Boateng and Franck Ribery are also injured, with defender German Pezzella suspended.
Nikola Milenkovic and Martin Caceres can play, as they are suspended for this weekend’s Serie A trip to Juventus.
Chiesa is partnered by Dusan Vlahovic, who scored the last-gasp equaliser when these teams met recently in Serie A, rather than Patrick Cutrone.
Dalbert is on loan from Inter and faces his former club.
Inter: Handanovic; Godin, Ranocchia, Bastoni; Candreva, Vecino, Barella, Young; Alexis Sanchez; R Lukaku, Lautaro Martinez
Inter bench: Padelli, Berni, Moses, Borja Valero, Dimarco, Eriksen, Esposito, Pirola, Agoume, D’Ambrosio, Biraghi, Skriniar
Fiorentina: Terracciano; Milenkovic, Ceccherini, Caceres; Lirola, Benassi, Badelj, Pulgar, Dalbert; Chiesa, Vlahovic
Fiorentina bench: Dragowski, Brancolini, Ranieri, Sottil, Maxi Olivera, Eysseric, Ghezzal, Montiel, Venuti, Zurkowski, Cutrone, Terzic
Ref: Doveri