Cagliari have Diego Godin initially on the bench, as Lazio begin their Serie A season with Ciro Immobile and Joaquin Correa.
It kicks off at the Sardegna Arena at 17.00 UK time (16.00 GMT).
The Sardinians opened with a 1-1 draw away to Cagliari under new coach Eusebio Di Francesco, but are still a team in flux following numerous changes.
There was another over the last couple of days, as Godin arrived from Inter and the veteran Uruguay international defender is initially on the bench in favour of Ragnar Klavan.
Gaston Pereiro and Filip Bradaric are still out of action, but Riccardo Sottil completes the trident with Giovanni Simeone and Joao Pedro.
Lazio saw their Week 1 fixture against Atalanta postponed to September 30 because La Dea needed extra time following their Champions League quarter-final efforts.
This is therefore the season opener for Simone Inzaghi, who made no secret of his frustration at the lack of genuine reinforcements from the transfer market this summer.
Considering they will be in the Champions League, strength in depth will be more important than ever.
Senad Lulic, Luiz Felipe and Denis Vavro are injured, with new striker Vedat Muriqi unavailable.
It means no new faces in the starting XI, relying yet again on Sergej Milinkovic-Savic, Luis Alberto, Correa and Capocannoniere Immobile.
Cagliari: Cragno; Faragò, Walukiewicz, Klavan, Lykogiannis; Nandez, Marin, Rog; Sottil, Simeone, Joao Pedro
Lazio: Strakosha; Patric, Acerbi, Radu; Lazzari, Milinkovic-Savic, Leiva, Luis Alberto, Marusic; Correa, Immobile