Simone Inzaghi can’t really rotate his Lazio squad much ahead of the Champions League, as Sampdoria pose a danger with Keita Balde Diao.
It kicks off at the Stadio Olimpico at 14.00 GMT.
The Biancocelesti have a huge Champions League Round of 16 clash coming up with Bayern Munich, but Inzaghi’s options are limited.
That’s because Luiz Felipe and Stefan Radu just had surgery and are out for some time, with Thomas Strakosha also injured, plus Manuel Lazzari and Wesley Hoedt suspended.
It means Senad Lulic gets his first start in over a year, since February 5, 2020.
January signing Mateo Musacchio therefore starts in defence, with Joaquin Correa chosen ahead of Vedat Muriqi.
Ciro Immobile continues to lead the way with support from Luis Alberto and Sergej Milinkovic-Savic.
This could be a very special game for Inzaghi, as he currently has 99 Serie A victories under his belt.
Sampdoria already beat Lazio once this season when they were distracted by the Champions League and are eager to make it a rare double, especially as they haven’t won at the Olimpico since January 2005.
Coach Claudio Ranieri finally has a full squad at his disposal, with Manolo Gabbiadini back on the bench, and can count on former Lazio striker Keita Balde Diao, who famously left the Olimpico in bitter circumstances following a contract dispute with President Claudio Lotito.
Fabio Quagliarella gets the nod as his strike partner, with Gaston Ramirez in support.
Antonio Candreva is another ex-Lazio player who has rediscovered his form at Samp.
Lazio: Reina; Patric, Acerbi, Musacchio; Marusic, Milinkovic-Savic, Leiva, Luis Alberto, Lulic; Correa, Immobile
Sampdoria: Audero; Ferrari, Yoshida, Colley, Augello; Candreva, Ekdal, Silva, Ramirez; Keita, Quagliarella